Heterozygous diploid and interspecies SCRaMbLEing

Single-cell isolations of diploid heterozygous Escherichia coli.

Diploid Heterozygous Hybrids from Matings between Escherichia Coli and Salmonella Typhosa

An Hfr strain of E. coli K-12 has been shown to mate at low frequency with a number of strains of S. typhosa. The hybrids, selected as lactose positives, retained all other antigenic and biochemical manifestations of the S. typhosa parent. A Lac(+) hybrid, S. typhosa strain 643L(+) was remated with the E. coli Hfr and plated on minimal media containing L-arabinose, D-xylose, L-rhamnose, or L-fu...

Segregants from a heterozygous diploid of Penicillium chrysogenum following different physical and chemical treatments.

Interspecies Sex and Taste

Different species of fruit flies share habitats but are believed to mate with each other only rarely. In this issue, Fan et al. show that interspecies mating is inhibited by the taste receptor Gr32a (Gustatory receptor 32a) and a neural circuit in which it functions.

Detection of heterozygous mutations in the genome of mismatch repair defective diploid yeast using a Bayesian approach.

DNA replication errors that escape polymerase proofreading and mismatch repair (MMR) can lead to base substitution and frameshift mutations. Such mutations can disrupt gene function, reduce fitness, and promote diseases such as cancer and are also the raw material of molecular evolution. To analyze with limited bias genomic features associated with DNA polymerase errors, we performed a genome-w...